polyclonal rabbit anti human ki 67 antigen  (Danaher Inc)

Journal: Frontiers in Oncology

Article Title: A Molecularly Characterized Preclinical Platform of Subcutaneous Renal Cell Carcinoma (RCC) Patient-Derived Xenograft Models to Evaluate Novel Treatment Strategies

doi: 10.3389/fonc.2022.889789

Figure Lengend Snippet: Histopathology, tumor growth characterization, and immunostainings (IHC) of representative PDX models from the RCC PDX panel. (A) Histological examination of patient and PDX tissue from the first (P1) and third (P3) PDX in vivo passage. FFPE tissue was used for 5-µm sections and standard H&E stainings. (B) Tumor growth characteristic of untreated control mice reflecting the heterogeneous biology of RCC regardless of the molecular phenotype. Data from twenty-two RCC PDX models utilized for drug testing studies as mean TV ± SEM, n = 3–6. (C) Representative IHC analyses from RCC in vivo passaged PDX tumors for Ki-67 (proliferation), CD31 or PECAM1 (blood vessels), and Pax2 and Pax8 (renal marker). The brown staining indicates the positivity for the respective markers within the tissue. Scale bar = 100 µm. (D) Exemplary RCC PDX growth curves showing individual TV growth characteristic during in vivo passaging (PT = primary tumor passage, P1–P4 indicate consecutive PDX tumor passages). (E) The gene expression-based ccRCC risk model (S3 score) was calculated for primary tumors and metastases from tissue of the Tübingen cohort collected for PDX generation. In 16 of the 24 cases shown, the PDX establishment was successful. The risk categories of the S3 score are represented by the background color. A high S3 score is equivalent to a good prognosis, while a low S3 score corresponds to a poor prognosis in terms of cancer-specific survival, indicating a correlation between S3 score and successful PDX establishment.

Article Snippet: Tissue incubation with primary antibodies— Ki-67 polyclonal rabbit anti-human Ki-67 antigen (Abcam #ab15580, Germany), CD31 polyclonal rabbit anti-human (Abcam #ab28364, Germany), and rabbit anti-human Pax2 and Pax8 (#PI593C002 and #PI924C002, DCS, Germany)—was conducted 45 min at RT.

Techniques: Histopathology, In Vivo, Control, Marker, Staining, Passaging, Expressing

Journal: Life

Article Title: Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development

doi: 10.3390/life11020164

Figure Lengend Snippet: Distribution of Ki-67 ( a ) pRb- ( b ), MMP-9- ( c ) and Bax- ( d ) positive cells in the epithelium (Ep), loose ectomesenchyme condensations (lec), loose non-condensing ectomesenchyme (lnc) in formation of the secondary palate (7th–12th week). Data are shown as mean ± SD. Significant differences (Kruskal–Wallis) indicated by * p < 0.05, ** p < 0.001, *** p < 0.0001.

Article Snippet: Slides were incubated with protein block for 30 min following overnight incubation at 4 °C with an appropriate primary antibody mixture—mouse monoclonal anti-human-MMP-9 (MA5-14228), rabbit monoclonal anti-human pRb (ab173289, Abcam, UK), rabbit polyclonal anti-human Ki-67 antigen (AB9260, Chemicon, Temecula, CA, USA), and anti-mouse Bax antigen (AB 2915, Chemicon, Temecula, CA, USA).

Techniques:

Journal: Life

Article Title: Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development

doi: 10.3390/life11020164

Figure Lengend Snippet: Staining intensity to specific antibodies in formation of the secondary palate during the 7th, 9th, and 12th weeks of development.

Article Snippet: Slides were incubated with protein block for 30 min following overnight incubation at 4 °C with an appropriate primary antibody mixture—mouse monoclonal anti-human-MMP-9 (MA5-14228), rabbit monoclonal anti-human pRb (ab173289, Abcam, UK), rabbit polyclonal anti-human Ki-67 antigen (AB9260, Chemicon, Temecula, CA, USA), and anti-mouse Bax antigen (AB 2915, Chemicon, Temecula, CA, USA).

Techniques: Staining

Journal: Life

Article Title: Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development

doi: 10.3390/life11020164

Figure Lengend Snippet: Transversal section through the secondary palate of 7 weeks ( a ), 9 weeks ( b ) and 12 weeks ( c ) old human embryo: pRb- (green) and Ki-67- (red) positive cells (arrows) can be seen in the epithelium (Ep), loose non-condensing ectomesenchyme (lnc), and loose ectomesenchymal condensations (lec). Some cells are co-expressing both markers (arrowheads). Blue nuclear staining (DAPI). Scale bar 25 µm.

Article Snippet: Slides were incubated with protein block for 30 min following overnight incubation at 4 °C with an appropriate primary antibody mixture—mouse monoclonal anti-human-MMP-9 (MA5-14228), rabbit monoclonal anti-human pRb (ab173289, Abcam, UK), rabbit polyclonal anti-human Ki-67 antigen (AB9260, Chemicon, Temecula, CA, USA), and anti-mouse Bax antigen (AB 2915, Chemicon, Temecula, CA, USA).

Techniques: Expressing, Staining

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Serial sections through an Em Ca with a morule. Boxes with solid and dotted lines indicate magnified morule and Sur-Ca regions, respectively. Staining is H&E (A, H, O), and for β-catenin (B, I, P), cyclin D1 (C, J, Q), p53 (D, K, R), and p21WAF1 (E, L, S), Ki-67 (F, M, T), and PML (G, N, U). Note apparent differences in nuclear staining patterns for β-catenin (P, focal nuclear staining in the glandular component indicated by the arrow), cyclin D1, p53, and p21WAF1, and Ki-67 immunoreactivity between morule and Sur-Ca lesions. G, N, U: There is relatively weak PML immunoreactivity in morule (N) and the Sur-Ca (U), in contrast to the reactions for infiltrating lymphocytes and stromal cells (arrows). Original magnifications: ×200 (A–G); ×400 (H–U).

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques: Staining

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Serial sections through an Em Ca with an area of squamous metaplasia (SqM). Boxes with solid and dotted lines indicate magnified regions of a SqM area and Sur-Ca, respectively. Staining is H&E (A, H, O), and for β-catenin (B, I, P), cyclin D1 (C, J, Q), p53 (D, K, R), and p21WAF1 (E, L, S), Ki-67 (F, M, T), and PML (G, N, U). Note sporadic nuclear immunoreactivity for β-catenin (I, positive cells are indicated by arrows), cyclin D1, p53, p21WAF1, and Ki-67 in the SqM areas, as well as the Sur-Ca, with the exception of Ki-67 in the latter. G, N, U: There is strong PML immunoreactivity in the SqM areas, as well as infiltrating lymphocytes and stromal cells (U, indicated by arrows). Original magnifications: ×200 (A–G); ×400 (H–U).

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques: Staining

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Relations of LIs or immunohistochemistry scores for nuclear β-catenin, cyclin D1, p53, p21WAF1, Ki-67, and PML among morules, areas of squamous metaplasia (SqM), and the surroundings or glandular carcinomas without SqD (Sur-Ca and Ca w/o SqD). The data are means ± SD values.

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques: Immunohistochemistry

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Relations of LIs or immunohistochemistry scores for nuclear β-catenin, cyclin D1, p53, p21WAF1, Ki-67, and PML with the β-catenin gene status (P, mutation positive; N, negative) in areas of SqD (A) and Sur-Ca categories (B). mor, Morule; SqM, squamous metaplasia; β-Cat Mut, β-catenin gene mutation. The data are means ± SD values.

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques: Immunohistochemistry, Mutagenesis

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Correlations among β-Catenin, Cyclin D1, p53, p21WAF1, Ki-67, and PML for Areas of Squamous Differentiation and the Surrounding Glandular Endometrial Carcinomas

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques:

Journal:

Article Title: ?-Catenin Simultaneously Induces Activation of the p53-p21WAF1 Pathway and Overexpression of Cyclin D1 during Squamous Differentiation of Endometrial Carcinoma Cells

doi:

Figure Lengend Snippet: Proposed molecular mechanisms underlying development of SqD in Em Cas. β-Catenin gene mutations and other factors, including endogenous progesterone, lead to accumulation of nuclear β-catenin and aberrant activation (by β-catenin together with TCF4) of the p14ARF-p53-p21WAF1 pathway that suppresses cell proliferation and induces senescence. Other factors, including progesterone, may also be required for development of squamoid phenotypes of Em Ca cells, with β-catenin as the initial signal for the trans-differentiation. Overexpression of cyclin D1 induced by β-catenin and PML expression may also play an important role in formation of morules or areas of squamous metaplasia (SqM).

Article Snippet: Briefly, slides were heated in 10 mmol/L citrate buffer (pH 6.0) for two 10-minute cycles using a microwave oven and then incubated overnight at 4°C with anti-β-catenin mouse monoclonal (Transduction Laboratories, Lexington, KY), anti-cyclin D1 mouse monoclonal (DAKO), anti-p53 mouse monoclonal (DO7; Novocastra Lab. Ltd., Newcastle, UK), anti-p21WAF1 mouse monoclonal (WAF1; Calbiochem, Cambridge, MA), anti-human Ki-67 antigen rabbit polyclonal (DAKO), or anti-PML rabbit polyclonal (Chemicom, Temecula, CA) antibodies.

Techniques: Activation Assay, Over Expression, Expressing